Yadda za a zabi PCR/qPCR abubuwan amfani?

PCR hanya ce mai mahimmanci kuma mai tasiri don haɓaka kwafi ɗaya na jerin DNA da aka yi niyya zuwa miliyoyin kwafi a cikin ɗan gajeren lokaci. Sabili da haka, abubuwan amfani da filastik don halayen PCR dole ne su kasance ba tare da gurɓatawa da masu hanawa ba, yayin da suke da inganci mai inganci wanda zai iya tabbatar da mafi kyawun tasirin PCR. Ana samun abubuwan amfani da filastik na PCR a cikin nau'ikan girma da tsari iri-iri, kuma sanin halayen samfuran da suka dace zai taimaka muku wajen zaɓar samfuran filastik da suka dace don ingantaccen bayanan PCR da qPCR.

Haɗin kai da Halayen abubuwan amfani da PCR

1.Kayayyaki
Abubuwan amfani da PCR yawanci ana yin su ne da polypropylene, wanda ba shi da isasshen ƙarfi don jure wa saurin canjin zafin jiki yayin hawan keken zafi da rage ɗaukar abubuwa masu amsawa don tabbatar da kyakkyawan sakamako na PCR.Don ƙara tabbatar da daidaiton tsari-zuwa-tsari a cikin tsabta da daidaituwa. Likita-matakin likita, ya kamata a yi amfani da albarkatun albarkatun polypropylene masu inganci yayin samarwa da kera su a cikin ɗaki mai tsabta na Class 100,000. Dole ne samfurin ya kasance ba tare da gurɓatar ƙwayoyin cuta da DNA ba don guje wa tsoma baki tare da tasirin gwajin haɓaka DNA.

2.Launi
Farashin PCRkumaPCR tubegabaɗaya ana samunsu cikin m da fari.

• Zane-zanen kauri na bango na uniform zai samar da daidaitaccen canjin zafi don samfuran amsawa.

• Babban ikon gani don tabbatar da mafi kyawun watsa siginar haske da ƙarancin murdiya.

• A cikin gwaje-gwajen qPCR, farin rami ya hana jujjuya siginar kyalli da kuma ɗaukarsa ta tsarin dumama.

3.Format
Farashin PCR "skirt" yana kewaye da allo. Siket ɗin yana ba da mafi kyawun kwanciyar hankali don tsarin pipetting lokacin da aka gina tsarin amsawa, kuma yana ba da mafi kyawun ƙarfin injin yayin jiyya ta atomatik. PCR farantin za a iya raba babu siket, rabin siket da cikakken siket.

• Farantin PCR mara siket da ya ɓace a kusa da farantin, kuma ana iya daidaita wannan nau'in farantin amsa don yawancin kayan aikin PCR da kayan aikin PCR na ainihi, amma ba don aikace-aikacen sarrafa kansa ba.

• Farantin PCR da aka yi da siket yana da ɗan gajeren gefen gefen farantin, yana ba da isasshen tallafi yayin bututu da ƙarfin injina don sarrafa mutum-mutumi.

• Cikakken farantin PCR yana da gefen da ke rufe tsayin farantin. Wannan nau'in faranti ya dace da ayyukan sarrafa kansa, wanda zai iya zama amintaccen daidaitawa da karko. Cikakken siket kuma yana haɓaka ƙarfin injina, yana mai da shi manufa don amfani da mutum-mutumi a cikin aiki mai sarrafa kansa.

Ana samun bututun PCR a cikin bututu guda ɗaya da 8-strips, waɗanda suka fi dacewa da ƙarancin kayan aiki na PCR/qPCR mai ƙarancin zuwa matsakaici. An tsara murfin lebur don sauƙaƙe rubuce-rubuce, kuma babban amincin watsa siginar walƙiya na iya zama mafi dacewa ta hanyar qPCR.

• Bututu guda ɗaya yana ba da sassauci don saita ainihin adadin halayen. Don mafi girma juzu'in amsawa, ana samun bututu guda ɗaya a cikin girman 0.5 ml.
• Tushen 8-strips tare da iyakoki yana buɗewa kuma yana rufe bututun samfurin da kansa don hana samfurin.

4.hatimi
Rufin bututu da fim ɗin rufewa dole ne gaba ɗaya rufe bututu da farantin don hana ƙawancewar samfurin yayin zagayowar thermal. Za a iya samun hatimi mai ƙarfi ta hanyar amfani da kayan aikin fim da kayan aikin latsa.

• Rijiyoyin farantin PCR suna da tsayin daka a kusa da su. Wannan zane yana taimakawa wajen rufe farantin tare da fim ɗin rufewa don hana ƙazanta.
• Alamar haruffa akan farantin PCR zasu taimaka wajen gano rijiyoyin guda ɗaya da matsayi na samfuran da suka dace. Ana buga wasiƙun da aka bugu galibi da fari ko baki, kuma don aikace-aikace na atomatik, rubutun wasiƙa ya fi fa'ida don rufe gefuna na waje na farantin.

5.Flux aikace-aikace

Gwajin gwajin gwajin PCR / qPCR na iya ƙayyade irin nau'in kayan amfani da filastik ya kamata a yi amfani da su don mafi kyawun tasirin magani. Don aikace-aikacen shigar da ƙananan-zuwa-matsakaici, bututu gabaɗaya sun fi dacewa, yayin da faranti sun fi kyawu don gwaji na matsakaici-zuwa babba. An kuma ƙera faranti don yin la'akari da sassaucin raɗaɗi, wanda za'a iya raba shi zuwa tsiri ɗaya.

A ƙarshe, a matsayin muhimmin ɓangare na gina tsarin PCR, abubuwan amfani da filastik suna da mahimmanci don nasarar gwaje-gwaje da tattara bayanai, musamman a cikin aikace-aikacen aiki na matsakaici-zuwa-high.

A matsayin mai siyar da Sinanci na kayan amfani da filastik mai sarrafa kansa, Cotaus yana ba da tukwici na pipette, acid nucleic, nazarin furotin, al'adun tantanin halitta, ajiyar samfurin, hatimi, chromatography, da sauransu.

Danna kan taken samfur don duba cikakkun bayanan samfur na PCR masu amfani.

PCR Tube ;Farashin PCR