Gabatarwar Elisa Plate

Farashin ELISA: A cikin Enzyme Linked Immunosorbent Assay (ELISA), tsabta, maida hankali da rabo na antigens, kwayoyin cuta, kwayoyin da aka yi wa lakabi ko antigens da ke cikin maganin rigakafi; Nau'in buffer, maida hankali da Yanayi kamar ƙarfin ionic, ƙimar pH, zafin amsawa da lokaci suna taka muhimmiyar rawa. Bugu da ƙari, fuskar polystyrene mai ƙarfi (Polystyrene) a matsayin mai ɗaukar kaya kuma yana taka muhimmiyar rawa wajen tallan antigens, antibodies ko rukunin antigen-antibody.

Antigens, antibodies da sauran biomolecules suna adsorbed zuwa saman mai ɗauka ta hanyoyi daban-daban, gami da adsorption ta hanyar hydrophobic bonds, hydrophobic / ionic bonds, covalent bonding ta hanyar gabatarwar wasu ƙungiyoyi masu aiki irin su amino da ƙungiyoyin carbon, kuma ta hanyar gyaran fuska. . Hydrophilic bonding bayan jima'i.

TheElisa Plateza a iya raba rijiyoyin 48 da rijiyoyin 96 bisa ga adadin ramuka. Wanda aka saba amfani dashi shine rijiyar 96, wanda yakamata a zaba bisa ga mai karanta microplate.

Bugu da ƙari, akwai waɗanda za a iya cirewa da waɗanda ba za a iya cire su ba. Don waɗanda ba za a iya cirewa ba, an haɗa slats a kan dukkan allo tare. Sa'an nan kuma, ga waɗanda za a iya cirewa, an rabu da slats a kan allo, kuma allunan da aka raba Akwai ramuka 12 da ramuka 8. Gabaɗaya, ana amfani da faranti mai lakabin enzyme da ake iya cirewa a zamanin yau. Idan kun sayi wasu irin waɗannan faranti a baya, zaku iya siyan wasu tsiri masu alamar enzyme yanzu.

Ko da yake microplates da masana'antun daban-daban suka yi kama da juna gaba ɗaya, wasu ƙananan bayanai za su bambanta, kamar tsari, da dai sauransu. Wannan ya fi dacewa saboda suna buƙatar amfani da su tare da masu karatu na microplate daban-daban. Don haka, lokacin da kuke amfani da lokacin zabar siyan mai karanta microplate, yakamata ku yi la'akari da yadda mai karanta microplate ɗinku yayi kama. Amma gabaɗaya an daidaita su, wasu ne kawai za su bambanta. Saboda kayan aikin enzyme gabaɗaya polystyrene (PS), kuma polystyrene yana da ƙarancin kwanciyar hankali na sinadarai kuma ana iya narkar da su ta nau'ikan kaushi iri-iri (kamar hydrocarbons aromatic, halogenated hydrocarbons, da sauransu), kuma acid mai ƙarfi zai lalace. da alkalis. , ba mai jurewa ga maiko ba, kuma mai sauƙin canza launi bayan an fallasa shi zuwa hasken ultraviolet, don haka tabbatar da kula da waɗannan lokacin amfani daElisa Plate.