Me yasa ake amfani da PCR gabaɗaya daga PP?

"Kamar yadda muka sani, PCR wata hanya ce ta gwaji ta asali a cikin dakunan gwaje-gwaje na biochemical." Sakamakon gwajin ko da yaushe ba shi da gamsarwa, wanda zai iya zama saboda ɗan gurɓatawar abubuwan amfani da filastik PCR, ko tsangwama na gwaji wanda ya haifar da shigar da masu hanawa. Akwai wani dalili mai mahimmanci: Zaɓin kayan da bai dace ba kuma zai yi tasiri sosai akan sakamakon gwaji.

Akwai dalilai da yawa waɗanda ke shafar sakamakon gwajin PCR: yawanci akwai nau'ikan nau'ikan 7 masu zuwa.

1. Masu farawa: Maɓalli sune maɓalli ga takamaiman amsawar PCR, kuma ƙayyadaddun samfuran PCR ya dogara da matakin haɓakawa tsakanin masu haɓakawa da samfurin DNA;

2. Enzyme da maida hankalinsa;

3. Ingancin da maida hankali na dNTP;

4. Samfurin (jinin manufa) nucleic acid;

5. Mg2+ maida hankali;

6. Saitin zafin jiki da lokaci;

7. Yawan zagayowar;

8. Kayan aiki, kayan amfani, da sauransu.

Daga cikin abubuwa masu yawa masu tasiri, abubuwan da ake amfani da su na ɗaya daga cikin mahimman abubuwan da ba a manta da su ba.

Akwai nau'ikan iri da yawaPCR masu amfani: 8-tubes, ƙananan ƙananan ƙananan ƙananan tubes, daidaitattun bututu, ba-skirted, Semi-skirted, cikakken-skirted, da jerin PCR da qPCR faranti. Zaba yana da matukar wahala, kuma akwai matsalolin gama gari da yawa, bari mu dubi matsalolin da kowa ya zaɓaPCR masu amfani, da kuma yadda za a warware su?

Me yasaPCR masu amfanikullum sanya daga PP?

Amsa: Abubuwan amfani da PCR/qPCR gabaɗaya ana yin su ne da polypropylene (PP), saboda abu ne na halitta wanda ba shi da ƙarfi, saman ba shi da sauƙin mannewa ga ƙwayoyin halitta, kuma yana da juriya mai kyau da juriya na zafin jiki (ana iya yin autoclaved a digiri 121) ƙwayoyin cuta. kuma yana iya jure yanayin zafi yayin hawan keken zafi). Wadannan kayan yawanci suna cikin hulɗar kai tsaye tare da reagents ko samfurori, don haka kayan aiki masu kyau da fasaha masu kyau suna buƙatar zaɓar lokacin samarwa da shirye-shirye.