Aikace-aikacen gama gari na ELISA Plates

A matsayin kayan aiki na gwaji, ainihin tsarin tsarinFarashin ELISAshi ne jerin microplates dauke da m lokaci kayan (kamar sunadarai da antibodies). A cikin aikace-aikacen farantin ELISA, samfurin da za a gwada zai amsa tare da takamaiman kwayoyin halitta mai lakabin enzyme, sa'an nan kuma za a samar da canjin launi da ake iya gani ta hanyar ƙara matrix substrate, kuma za a ƙididdige abun ciki ko aiki na kwayoyin da aka yi niyya. ko kimantawa ta hanyar gano siginar abin sha ko kyalli. Waɗannan aikace-aikacen gama gari ne na faranti na ELISA a fagage daban-daban:

1. Protein Quantitative analysis: ELISA faranti za a iya amfani da su auna maida hankali da kuma aiki na sunadaran a cikin nazarin halittu samfurori kamar jini da kuma cell supernatants, samar da iko kayan aiki ga gano tumor alamomi, hepatitis cutar antibodies, myocardial rauni alamomi, da dai sauransu. da kuma taimaka wa likitoci a farkon ganewar asali da kuma tantance cututtuka.

2. Sa ido kan Cytokine: A cikin bincike na rigakafi,Farashin ELISAzai iya auna matakan cytokine a cikin al'adun sel masu girma ko ruwa mai nama, wanda ke taimakawa wajen fahimtar hanyoyin nazarin halittu kamar su amsawar rigakafi da kumburi, kuma yana da mahimmanci ga ci gaban sababbin jiyya da kwayoyi.

3. Binciken Nucleic acid: Ta hanyar faranti na ELISA, masana kimiyya za su iya ganowa da kuma nazarin abubuwan da ke ciki da ayyukan DNA ko RNA, ba da tallafi na bayanai don binciken nazarin halittu na kwayoyin halitta irin su maganganun kwayoyin halitta da tsarin tsarin kwayoyin halitta, da kuma kara inganta ci gaban filayen kamar maganin kwayoyin halitta. da gyaran kwayoyin halitta.

4. Enzyme bincike bincike: ELISA faranti na iya daidai auna aikin enzyme, taimaka masu bincike su fahimci aiki da tsarin tsarin enzymes a cikin kwayoyin halitta, da kuma samar da muhimman nassoshi ga bincike a cikin enzyme injiniya, na rayuwa injiniya da sauran filayen.

5. Binciken hulɗar tsakanin kwayoyin halitta:Farashin ELISAana iya amfani da su ba kawai don auna abubuwan da ke cikin kwayoyin halitta ba, har ma don nazarin hulɗar tsakanin kwayoyin halitta. Ta hanyar haɗa fasahohi irin su raɗaɗɗen plasmon sama da haɓakar haɓakar haɓakar makamashi, ana iya lura da tsarin ɗaure da rarrabuwar kawuna a cikin ainihin lokacin, samar da sabbin ra'ayoyi da hanyoyin ƙirar ƙwayoyi, hulɗar furotin da sauran bincike.