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Yadda za a Zaɓan Tasoshin Al'adun Kwayoyin Dama?

2024-11-29

Zaɓin kayan aikin al'adu masu dacewa yana da mahimmanci don tabbatar da ingantaccen haɓakar tantanin halitta da sakamakon gwaji. Lokacin zabar tasoshin al'adun tantanin halitta, yana da mahimmanci a yi la'akari da dalilai kamar nau'in tantanin halitta, takamaiman manufar al'adun ku, ma'aunin al'adun, nau'in matsakaicin al'ada, kayan da girman tasoshin, jiyya na sama, murfi don dacewa. musayar gas, da dacewarsu da kayan aikin dakin gwaje-gwajenku.

Anan akwai mahimman abubuwan da za a yi la'akari yayin zabar jirgin ruwan al'adun tantanin da ya dace

1. Zaɓi jirgin ruwan al'adar tantanin halitta bisa nau'in sel

Kwayoyin Maƙwabta

Waɗannan sel suna buƙatar ƙasa don haɗawa da bazuwa. Don sel masu mannewa, zaku buƙaci tasoshin da ke da fili wanda ke ba da isasshen sarari don haɗewar tantanin halitta da girma.

Misalai Filashin al'adar nama, jita-jita na petri, da faranti mai rijiyoyi da yawa.

Kwayoyin dakatarwa

Wadannan sel suna girma suna iyo a cikin matsakaici, don haka ba a la'akari da farfajiya.

MisalaiFilashin al'adun nama, flasks spinner, ko bioreactors don manyan al'adun dakatarwa.

2. Zaɓi jirgin ruwan al'adar tantanin halitta bisa girman (Ƙarfin Ƙarfin)

Ƙananan Al'adu

Don ƙananan gwaje-gwaje ko babban aikin nunawa, ƙananan jiragen ruwa sun dace.

Misalai Multi-rijiya faranti (6, 24, 96-cell al'ada faranti),Petri abinci, ko T25 flasks.

Al'adu Masu Girma

Idan kana buƙatar girma da yawa na sel, manyan tasoshin ko bioreactors sun fi kyau.

Misalai T75 da T175 flasks al'adun tantanin halitta, bioreactors, ko spinner flasks don al'adun tantanin dakatarwa.

3. Zabi jirgin ruwan al'adar tantanin halitta bisa la'akari da magani

Filayen Al'adar Nama Mai Magani

An riga an yi wa jiragen ruwa magani don haɓaka haɗewar tantanin halitta, wanda ya sa su dace da nau'ikan tantanin halitta. Wadannan gabaɗaya an lulluɓe su da abubuwa kamar collagen, fibronectin, ko wasu abubuwan haɗin matrix na waje.

Filayen da ba a yi Magani ba

Mafi dacewa don al'adun dakatarwa ko lokacin da sel ba sa buƙatar mannewa saman. Yawancin lokaci ana amfani da su don ƙwayoyin da ke girma da yardar rai a cikin matsakaici.

4. Zaɓi jirgin ruwan al'adar tantanin halitta bisa ga abu

Polystyrene da aka fi amfani da shi don daidaitattun aikace-aikacen al'adar salula. A bayyane yake, yana ba da izinin dubawa mai sauƙi na gani, kuma yana aiki da kyau ga duka biyun maƙwabta da sel masu dakatarwa.

Ana amfani da polycarbonate ko polypropylene don wasu aikace-aikacen bioreactor kuma don tasoshin da ke buƙatar ƙarin sassauci ko takamaiman jiyya na saman.

Gilashin da aka yi amfani da shi don daidaitaccen al'adun nama saboda farashi da karyewa, tasoshin gilashin na iya dacewa da takamaiman aikace-aikace ko al'adu masu girma.

5. Zabaabubuwan amfani da al'adun seldangane da ƙirar jirgin ruwa

Flasks

Don al'adar tantanin halitta, ana amfani da T-flasks (T25, T75, T150). Ƙaƙƙarfan shimfidar wuri yana ba da wuri mai kyau don haɗewar tantanin halitta da girma. Ana iya amfani da su duka biyun madaidaitan sel da al'adun dakatarwa idan an kiyaye yanayin da suka dace.

Petri Dishes

Na gama gari don ƙananan al'adu da kuma gwaje-gwajen da ke buƙatar lura, kamar ƙididdigar samuwar mulkin mallaka.

Multi-Well Plates

Waɗannan suna da amfani ga babban abin dubawa da ƙananan gwaje-gwaje. Faranti mai 6, 12, 24, 48,96, ko rijiyoyin 384 suna samuwa, kuma suna da kyau don ƙididdigar tushen tantanin halitta, sakin cytokine, gwajin magunguna, da sauran aikace-aikace masu girma.

Spinner Flasks

Ana amfani da shi don al'adun sel na dakatarwa, musamman a cikin ɗimbin girma inda tashin hankali ya zama dole don kula da haɓakar tantanin halitta kuma don gujewa cuɗanyar tantanin halitta.

Bioreactors

Don al'adun dakatarwa mai girma, masu amfani da bioreactor suna ba da damar ƙarin hadaddun sarrafawa akan yanayin muhalli (misali, pH, zafin jiki, oxygenation) kuma ana amfani da su don samarwa mai girma, kamar a masana'antar biopharmaceutical.

6. Zaɓi jirgin ruwan al'adar tantanin halitta bisa ga haifuwa da samun iska

Haihuwa

Tabbatar cewa jirgin ba ya cika ko kuma an haifuwa don gujewa gurɓatawa. Yawancin tasoshin al'adun kasuwanci an riga an sanya su, amma koyaushe bincika marufi.

Samun iska

Wasu tasoshin, kamar flasks, suna zuwa tare da huluna ko tacewa don ba da izinin musayar iska yayin hana kamuwa da cuta. Wannan yana da mahimmanci lokacin da ake haɓaka ƙwayoyin sel a cikin yanayi mai yawa.

7. Zaɓi jirgin ruwan al'adar tantanin halitta bisa dacewa da amfani

Autoclavable vs. Zazzagewa

Wasu tasoshin al'adu za a iya ƙera su don sake amfani da su (misali, kwalabe gilashi, wasu filaye na filastik), yayin da wasu ke amfani da su guda ɗaya kuma ana iya zubar da su (misali, jita-jita na petri filastik, faranti mai yawa).

Gudanarwa da sufuri

Yi la'akari da sauƙi na canja wurin sel tsakanin tasoshin. Misali, faranti mai rijiyoyi da yawa na iya buƙatar faranti na musamman don sauƙin sarrafawa da kayan aiki irin su pipettors masu sarrafa kansu.

8. Zaɓi jirgin ruwan al'adar tantanin halitta bisa ga al'ada matsakaici girma

Zaɓi jirgin ruwa wanda zai iya ɗaukar adadin matsakaicin al'adu da ake so ba tare da ɓata albarkatu ba. Idan aiki tare da al'ada mai girma, manyan flasks ko bioreactors na iya zama dole, yayin da ƙananan kundin sun dace da jita-jita na al'adun tantanin halitta ko faranti.

9. Zaɓi jirgin ruwan al'adar tantanin halitta bisa la'akari da farashi

Za'a iya zubarwa vs. Maimaituwa

Tasoshin filastik da ake zubar da su suna da tsada kuma suna rage haɗarin kamuwa da cuta, amma suna iya yin tsada don manyan ayyuka. Tasoshin gilashin da za a sake amfani da su suna da farashi mai girma na gaba amma ana iya haifuwa da sake amfani da su sau da yawa, yana mai da su tattalin arziki don amfani na dogon lokaci.

Ƙarfin Ƙarfafawa

Tabbatar cewa girman jirgin ruwa ya dace don guje wa ɓarna na kayan, musamman lokacin amfani da kafofin watsa labarai masu tsada ko reagents.

10. Zaɓi jirgin ruwan al'adar tantanin halitta bisa takamaiman buƙatun aikace-aikacen

Hoto

Idan kana buƙatar ganin sel a ƙarƙashin na'urar gani, zaɓi tasoshin tare da bayyanannun kayan gani da ma'auni masu dacewa don saitin hotonku (misali, faranti mai rijiyoyi masu yawa don babban abun ciki ko jita-jita na gilashin ƙasa don hoton tantanin halitta).

Tashin hankali mai sarrafawa

Don al'adun sel masu dakatarwa, yi la'akari da flasks spinner ko bioreactors waɗanda ke ba da tashin hankali don kiyaye sel daidai da dakatarwa.

Kammalawa

Zaɓin madaidaicin jirgin ruwan al'adar tantanin halitta yana buƙatar daidaita abubuwa da yawa, gami da nau'in tantanin halitta, sikelin al'ada, dacewa da kayan aiki, da takamaiman buƙatun gwaji. Kwayoyin da ke mannewa za su buƙaci filaye waɗanda ke haɓaka haɗe-haɗe, yayin da sel masu dakatarwa suna fa'ida daga girma da tashin hankali. Don ƙaramin aiki, faranti mai rijiyoyi da yawa ko T-flasks na iya wadatar, yayin da manyan al'adu na iya buƙatar filayen kashin baya ko na'urorin bioreactors. Koyaushe tabbatar da cewa tasoshin suna saduwa da haifuwarku da buƙatun kulawa, kuma kuyi la'akari da ingancin farashi dangane da amfanin ku.

Ta yin la'akari da waɗannan abubuwan, za ku iya zaɓar jirgin ruwa mafi kyau wanda ke ba da yanayi masu dacewa don al'adun cell ɗin ku da kuma burin gwaji.

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