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Me yasa al'adar tantanin halitta ke sanya jajayen ƙwayoyin jini da farko?

2022-12-23

Gabatarwa ta asali
Erythrocyte lysate yana daya daga cikin mafi sauki da sauki hanyoyin cire jajayen kwayoyin halitta, wato raba jajayen kwayoyin jini da lysate, wanda baya lalata kwayoyin halitta kuma yana iya cire jajayen kwayoyin jini gaba daya. Lysate cleavage hanya ce mai sauƙi mai cire kwayar jini mai sauƙi, wanda aka fi amfani dashi don rabuwa da tsarkakewar ƙwayoyin nama da aka tarwatsa ta hanyar narkewar enzyme, rabuwa da tsarkakewar lymphocytes, da kuma kawar da jajayen kwayoyin jini a cikin gwaje-gwaje na furotin nama da tsakiya. cire acid. Kwayoyin nama da aka samu ta hanyar lysate na sel ja ba su ƙunshi jajayen ƙwayoyin jini ba, kuma ana iya ƙara amfani da su don al'ada ta farko, haɗuwa da kwayar halitta, cytometry mai gudana, rabuwa da cirewar nucleic acid da furotin, da dai sauransu.

Umarnin don amfani
Samfurin kwayar halitta
1. Sabbin kyallen takarda an narkar da su ta hanyar pancreas/enzyme ko collagenase kuma an tarwatsa su cikin dakatarwar tantanin halitta guda ɗaya, kuma an watsar da supernatant ta hanyar centrifugation.

2. Ɗauki ELS lysate daga firiji a 4â, ƙara ELS lysate zuwa tantanin halitta precipitate a cikin wani rabo na 1: 3-5 (ƙara 3-5ml na lysate zuwa 1ml na cell compacted), a hankali busa da Mix.

3. Centrifuge a 800-1000rpm na minti 5-8 kuma zubar da ruwa mai tsabta na sama.

4. An tattara ɓangaren da aka haɗe kuma an saka shi tare da maganin Hank ko maganin al'ada mara amfani na sau 2-3.

5, idan fashewar ba ta cika ba za a iya maimaita matakai na 2 da 3.

6. Kwayoyin dakatarwa don gwaje-gwaje na gaba; Idan an fitar da RNA, yana da kyau a yi haka a cikin maganin da aka shirya daga Mataki na 4 ta amfani da ruwan DEPC

Kwayoyin jajayen jini suna da gajeriyar zagayowar rayuwa, kwanaki 120 kacal, amma suna haifuwa da sauri da sauri, kuma a wannan yanayin sun fi iya rarraba kwayoyin halitta, kuma su ne mafi saurin rarraba kwayoyin halitta, don haka wannan tantanin yana da matukar daraja. don haka yana da matukar amfani ga al'adun tantanin halitta. Abu ne mai sauqi qwarai, ba shi da wani qwayoyin qwayoyin qwayoyin halitta a cikinsa, sai dai membranes cell da proteins.

A baya:Menene ayyukan kit ɗin ELISA?

Na gaba:Abubuwan buƙatun reagent da wakilcin asali na maida hankali.